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Saturday, April 13, 2013

Testing antibotics on bacteria

Introduction

Problem

        To determine the effect of ten different antibiotics on deuce different types of bacteria. I will trial six antibiotics on Escherichia coli, and six

antibiotics on Bacillus subtilis. On Escherichia coli I will rill tetracycline, chloramphenicol, furadantin, nalidixic acid, triple sulfa, and kanamycin. On Bacillus subtilis I will test streptomycin, erythromycin, novobiocin, tetracycline, chloramphenicol, and penicillin. As a side observation, I would too equal to see if Bacillus subtillis shows resistance to penicillin. The use of penicillin is being cut down because of the resistance many types of bacteria are developing against it.

        

Hypothesis

        My dead reckoning is that penicillin will inhibit the most growth against Bacillus subtilis, and that tetracycline will stop Escherichia coli more effectively than the others.

Rationale

        I facial expression that this experiment is valid because it shows how different antibiotics react to different types of bacteria. It also points out the fact that not altogether antibiotics work the kindred or that they work at all on all types of bacteria.

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Materials

1 bottle of tryptic soy agar-agar

1 culture of Escherichia coli

1 culture of Bacillus subtilis

4 stereotypic petri yellowish pinkes

1 pack of test discs

2 unfruitful pipettes

2 tubes of physiological saline

1 forceps

1 wax pencil

2 sterile swabs

Method

1. Loosen the cap of the tryptic soy agar to allow it to vent. localise the bottle in a water bath at 100 degrees Centigrade so that the water level reached the level of the medium. The agar will melt in about 20 minutes.

2. piecemeal cool the medium to about 45 degrees Centigrade by allow the water bath cool. Then let the medium mould for 10 minutes.

3. Pour two plates for each species of bacteria. The agar should be approximately 5 mm deep. Cover each petri dish immediately after poring...

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